evaluation of lipid peroxidation as an indirect measure of oxidative stress in seminal plasma
نویسندگان
چکیده
background: it has been proposed that oxidative stress plays an important role in male infertility. the aims of this study were to compare seminal plasma levels of 15-f2t-isoprostane (8-iso-pgf2?), malondialdehyde (mda), and total (sum of free and bound) homocysteine (thcy) in normozoospermic vs. asthenozoospermic men, and to examine the relationships between thcy and lipid peroxidation products. materials and methods: the study was a case-control study with a simple random sampling. the case group consisted of 15 asthenozoospermic males. this group was compared with 15 normozoospermic men. seminal plasma levels of 15-f2t-isoprostane and thcy were measured using commercially available enzyme immunoassay (eia) kits. mda levels were determined by the thiobarbituric acid (tba) assay. the mann-whitney u test was used to compare two groups. coefficients of correlation were calculated using spearman’s correlation analysis. all hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. results: mda levels were lower in asthenozoospermic subjects than in control subjects (0.72±0.06 µm vs. 0.40±0.06 µm; p<0.05). no differences were seen in 15-f2t-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml; p>0.05). interestingly, thcy levels were slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 µm vs. 4.8±0.52µm). sperm motility was inversely correlated with seminal plasma 15-f2t-isoprostane and mda levels, respectively (p<0.05). conclusion: seminal plasma levels of 15-f2t-isoprostane and thcy showed no significant differences between normozoospermic and asthenozoospermic men. sperm motility correlated inversely with seminal plasma levels of 15-f2t-isoprostane and mda. no relationship was found between thcy and lipid peroxidation. however, higher sample size is required to confirm these findings.
منابع مشابه
Lipid peroxidation products as oxidative stress biomarkers.
Oxidative stress induced by reactive oxygen and nitrogen species has been implicated in the pathogenesis of various disorders and diseases. Biomarkers are needed for assessment of oxidative stress status in vivo and also for health examination, diagnosis at early stage, prognosis, safe and efficient drug development, and evaluation of efficacy of drugs, foods, beverages, and supplements. Lipids...
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عنوان ژورنال:
international journal of reproductive biomedicineجلد ۲، شماره ۱، صفحات ۳۴-۰
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